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pcg vector  (Addgene inc)


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    Addgene inc pcg vector
    Pcg Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcg vector/product/Addgene inc
    Average 93 stars, based on 7 article reviews
    pcg vector - by Bioz Stars, 2026-03
    93/100 stars

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    (A) Localization of GFP-18E2, GFP-6E2 and GFP-ΔTAD in HaCaT cells infected with increasing m.o.i. of the corresponding recombinant adenoviruses (Ad). (B) Left panel: AdGFP-18E2 and AdGFP-ΔTAD-infected cells (m.o.i. 200) labeled with MitoTracker red. Right panel: Cells transfected with untagged HPV-18 E2 and ΔTAD expression vectors, labeled with anti-HPV-18 E2 antibody and MitoTracker red. (C) Time-lapse microscopy analyses of Saos-2 living cells (osteosarcoma cells used here for their large cytoplasm) infected with AdGFP-18E2 (m.o.i. 200), labeled with MitoTracker red and Hoechst 33342.

    Journal: PLoS ONE

    Article Title: Localization of HPV-18 E2 at Mitochondrial Membranes Induces ROS Release and Modulates Host Cell Metabolism

    doi: 10.1371/journal.pone.0075625

    Figure Lengend Snippet: (A) Localization of GFP-18E2, GFP-6E2 and GFP-ΔTAD in HaCaT cells infected with increasing m.o.i. of the corresponding recombinant adenoviruses (Ad). (B) Left panel: AdGFP-18E2 and AdGFP-ΔTAD-infected cells (m.o.i. 200) labeled with MitoTracker red. Right panel: Cells transfected with untagged HPV-18 E2 and ΔTAD expression vectors, labeled with anti-HPV-18 E2 antibody and MitoTracker red. (C) Time-lapse microscopy analyses of Saos-2 living cells (osteosarcoma cells used here for their large cytoplasm) infected with AdGFP-18E2 (m.o.i. 200), labeled with MitoTracker red and Hoechst 33342.

    Article Snippet: Transfection with pCG expression vectors for untagged HPV-18 E2 or ΔTAD were performed using Lipofectamine (Invitrogen).

    Techniques: Infection, Recombinant, Labeling, Transfection, Expressing, Time-lapse Microscopy

    (A) Left panel: Western-blot analyses after fractionation of extracts from HaCaT cells infected with AdGFP-ΔTAD, AdGFP-18E2, and AdGFP-6E2. Nuc: nuclear fraction, Cyt: cytoplasmic fraction, Mito: crude mitochondrial fraction after Percoll gradient. Porin, β-tubulin and cdc27 were used as markers of mitochondria, cytoplasm and nucleus respectively. Right panel: The crude mitochondrial fraction was submitted to alkali extraction. Tot: total crude mitochondria before alkali extraction. Pellet: pellet after alkali extraction containing membrane-bound proteins, Sol: supernatant after alkali extraction containing soluble proteins. (B) Immunohistochemistry showing localization of HPV-18 E2 in CIN II (left panels) and HPV-6 E2 in condyloma (right panels) using the anti-HPV-16 E2 antibody. The lower panels are high magnifications of upper panels. The first upper panel shows labeling of a HPV-negative normal cervical epithelium with the same anti-HPV-16E2 antibody performed in the same conditions as the HPV-positive samples.

    Journal: PLoS ONE

    Article Title: Localization of HPV-18 E2 at Mitochondrial Membranes Induces ROS Release and Modulates Host Cell Metabolism

    doi: 10.1371/journal.pone.0075625

    Figure Lengend Snippet: (A) Left panel: Western-blot analyses after fractionation of extracts from HaCaT cells infected with AdGFP-ΔTAD, AdGFP-18E2, and AdGFP-6E2. Nuc: nuclear fraction, Cyt: cytoplasmic fraction, Mito: crude mitochondrial fraction after Percoll gradient. Porin, β-tubulin and cdc27 were used as markers of mitochondria, cytoplasm and nucleus respectively. Right panel: The crude mitochondrial fraction was submitted to alkali extraction. Tot: total crude mitochondria before alkali extraction. Pellet: pellet after alkali extraction containing membrane-bound proteins, Sol: supernatant after alkali extraction containing soluble proteins. (B) Immunohistochemistry showing localization of HPV-18 E2 in CIN II (left panels) and HPV-6 E2 in condyloma (right panels) using the anti-HPV-16 E2 antibody. The lower panels are high magnifications of upper panels. The first upper panel shows labeling of a HPV-negative normal cervical epithelium with the same anti-HPV-16E2 antibody performed in the same conditions as the HPV-positive samples.

    Article Snippet: Transfection with pCG expression vectors for untagged HPV-18 E2 or ΔTAD were performed using Lipofectamine (Invitrogen).

    Techniques: Western Blot, Fractionation, Infection, Immunohistochemistry, Labeling